iBright Instrument e-signature workflows requires all signatures to be obtained before exiting the e-signature workflow. Recently, a truly quantitative and more sensitive western blotting technology has been developed whereby the level of fluorescence measured is directly related to the quantity and expression of a single protein within a sample: quantitative fluorescent western blotting (QFWB). MUC2, p-PKC/ and p-Erk1/2 in TS-treated SL174T cells were quantified by using Image J program and statistical analysis was performed. When all signatures are obtained, the file will lock. Note: iBright Analysis Software-Secure allows partially signed files to be shared across user computers in partially signed state. Samples were analyzed by Western blot, using antibodies against the N-terminal epitope ARGS and the G3 domain, and fragments were quantified using a digital. When all signatures are obtained, the file will be fully signed and locked. Select the applicable role and enter credentials. Required signatures and roles will be listed. In e-signature workflow, select the applicable e-signature meaning. Use of capillary Western immunoassay (Wes) for quantification of dystrophin levels in skeletal muscle of healthy controls and individuals with Becker and Duchenne muscular dystrophy - PMC Journal List PLoS One PMC5895072 As a library, NLM provides access to scientific literature. A signature log will be added in e-signature summary table. Once all signatures are obtained, the status of the signature rule will be updated in the e-signature table for the corresponding version found in the audit tab from unsigned to signed. Signature requirements (number of signatures and role required) for the selected meaning will be listed in the table ( Figures 11a and 11b). When all the changes are done and committed, the file can be signed as per signature rules set in the SAE Administrator Console.
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